层析柱内径5mm 10mm 耐压50bar 耐有机

建议：第一次使用时请先清洗玻璃柱管和连接处，以便去除在生产过程中可能残留的物质。可选用常规的洗涤剂或实验室级去污剂来清洗，我们的玻璃柱管亦可高温消毒。

Cleaning

Recommendation: Before initial use, please clean the glass column tube and connections to remove any potential residues from the manufacturing process. Conventional detergents or laboratory-grade cleaning agents can be used for cleaning. Our glass column tubes can also be sterilized at high temperatures.

3、轻轻将柱床支撑的固定末端缓慢地垂直推进柱管，并保持整个过程在一条直线完成，一定要避免安装时有斜角以免损坏密封圈和玻璃管末端。

Installing the Fixed End

1.First, ensure the threads on the glass tube and the fixed end cap are free of debris and burrs to prevent damage during installation.

2.Thoroughly wet the O-ring seal with the mobile phase or a suitable solvent.

3.Gently and slowly insert the fixed end with the column bed support vertically into the column tube, maintaining alignment throughout the process. Avoid any angled insertion to prevent damage to the seal and the end of the glass tube.

Column Packing

After completing the steps above, the column can now be packed with chromatography media. As there are numerous types of media available, each with potentially different requirements, we recommend packing the column according to the media manufacturer's instructions.
We have pre-printed the column bed's cross-sectional area and height markings on the column tube to facilitate the calculation of bed volume and height during packing. The scale intervals are 10 mm. We have also printed the pressure resistance of the column tube. The scales we provide are for reference only.

注：通常在使用时柱床高度会略有下降，我们建议装柱时可将适配器的柱塞调节到略低于柱床沉降后的高度。

Adapter installation

1.First, ensure the glass tube and the fixed end cap are undamaged.

2.Close the column outlet to prevent the column bed from drying out.

3.Adjust the liquid level in the column tube to be at least 1-2 cm above the top of the column bed.

4.Screw the adjustment nut and the fixed cap on the adapter together down close to the frit.

5.Thoroughly wet the O-ring seal and the frit with water, ensuring no air bubbles are trapped in the frit.

6.Gently push the adapter vertically into the glass tube until the fixed cap can be screwed onto the glass column tube. 7.Ensure the entire operation is performed in a straight line.

8.After tightening the fixed cap, unscrew it by approximately 15°. Please avoid over-tightening the fixed cap.

Hold the fixed cap and slowly turn the adjustment nut counterclockwise to lower the adapter plunger to the required position within the column tube.
Note: The bed height often decreases slightly during use. We recommend adjusting the adapter plunger to a position slightly below the settled bed height after packing.

我们提供的管线上的1/4"-28接头是专用于连接柱管连接帽的。管线的另一端可自行添加与您层析系统所匹配的接头。

Column Connecting

The 1/4''-28 fittings on the tubing we supply are specifically designed for connecting to the column tube end cap. You can add a fitting compatible with your chromatography system to the other end of the tubing.

请避免O型-密封圈上残留有如IPA这类的有机溶剂，否则其会溶解密封圈，并使其粘连在柱管内壁。

Column Storage

If storing an empty column, simply rinse it thoroughly with clean water. If packed with media, it can also be stored in 20% ethanol.
Avoid leaving organic solvents (such as IPA) on the O-ring seals, as they can dissolve the seals and cause them to stick to the inner wall of the column tube.

4、 有时填料或使用的溶剂可能会残留在 O型密封圈上，导致适配器移动困难，此时可以将层析柱放入冰箱冷藏室数个小时，这样有助于移动适配器。

Precautions

1.The threaded parts of the glass tube are relatively fragile. During installation, try to avoid angled forces and handle gently.

2.When installing the adapter, ensure the O-ring is thoroughly wetted. Otherwise, friction between the seal and the column tube may increase, making axial compression difficult and potentially damaging the seal.

3.If replacing the frit, we recommend using our specialized tool for frit removal to avoid damaging the adapter end.

4.Sometimes media particles or used solvents may remain on the O-ring, making the adapter difficult to move. Placing the column in a refrigerator for several hours may facilitate adapter movement.